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Tracing the identity of novel brain biomarkers through the immune system
Author(s) -
Buonora John E,
Almeida Camila,
McCue Jeffrey,
Jacobowitz David,
Braga Maria,
Watson William,
Pollard Harvey,
Mueller Gregory P
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1001.3
Subject(s) - traumatic brain injury , biomarker , autoantibody , biomarker discovery , immune system , proteome , proteomics , medicine , neuroscience , concussion , bioinformatics , antibody , biology , immunology , biochemistry , poison control , gene , psychiatry , environmental health , injury prevention
Despite substantial effort, no biomarkers have been established for identifying mild traumatic brain injury (TBI). This investigation used the humoral immune response to TBI as a pathway for the discovery of novel, brain‐specific proteins that may serve as early biomarkers for mild TBI. The appearance of TBI‐induced autoantibodies was documented in rats seven days following controlled cortical impact. Serum from control and TBI animals was used to interrogate western blots of the entire rat brain proteome fractionated on large, 2‐D gels. Proteins revealed by autoreactive IgGs were mapped to corresponding features on Commassie stained protein gels. The candidate biomarkers were excised and identified proteomically by tryptic mass finger printing using MALDI‐TOF mass spectral analysis. Identified candidate TBI biomarkers included the brain specific isoform of creatine kinase (CK‐B), a recognized biomarker for TBI. Additional candidates included CRIMP‐2 and alpha‐internexin. Our success in confirming the identify of a recognized TBI biomarker (CK‐B) supports the potential validity of novel TBI candidates discovered by this approach. Funding: DoD in the Center for Neuroscience and Regenerative Medicine.