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Synchrotron radiation exposure of mice head leads to reduction of spleen weight, but not testicular weight, nor spleen cytokine levels
Author(s) -
Shao Jiaxiang,
Liu Yewei,
Liu Na,
Xia Weiliang,
Ying Weihai
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb706
Subject(s) - spleen , irradiation , nuclear medicine , medicine , chemistry , radiochemistry , physics , nuclear physics
Synchrotron radiation has wide biomedical applications yet one key issue is its safety concerns for use in medical procedures. In this study we carried out pilot experiments to explore the safety dosage by assessing cellular/organ damages in mice that underwent exposure of synchrotron radiation for varying durations (15, 10, 5, 3, 1 and 0 min, n=6 for each time point). ICR male mice (age: 6 wk) were anaesthetized with sodium pentobarbital before their head being exposed to radiation under the same parameters (32 keV, 1.5*E6~5*E7 mA chamber currency which is most widely used for Barium sulfate imaging in medical applications) in Shanghai Synchrotron Radiation Facility. The animals were euthanized 1 or 3 days post irradiation; spleen and testicles were removed, weighed and frozen in liquid nitrogen. It was found that paired testes weight did not change significantly 1 or 3 days afterwards with increasing duration of irradiation; however, 3 days afterwards spleen weight reduced significantly with 1 min exposure, and ~55% reduction was observed for 3 min and 5 min exposure group. Additional analysis of spleen cytokine levels (TNFα and IL6) by real‐time PCR found that no significant changes occurred either 1 or 3 days after irradiation. Further study is warranted to the understanding of synchrotron irradiation damage to other organs when the brain is the target irradiation site.