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GENETIC VARIANTS OF MICRO‐RNA 196a2 SEQUENCES CAN PREDICT SURVIVAL OF CHOLANGIOCARCINOMA
Author(s) -
Ko Kwang Hyun,
Kim Nam Keun
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb69
Subject(s) - microrna , medicine , gene , oncology , single nucleotide polymorphism , biology , survival analysis , gastroenterology , genotype , genetics
Background MicroRNAs (mir) are a new class of non‐protein‐coding, small RNAs that has function of tumor suppressors or oncogenes. They participate in diverse biological pathways and function as gene regulators. Only a few studies have been published about the association between microRNA polymorphism and malignant tumor. Aim We evaluated that the association between genetic variants of MicroRNA and survival of patients with cholangiocarcinoma. Patients and Method We enrolled 94 patients, who were diagnosed with cholangiocarcinoma between Jan.1999 to Nov. 2006 in Bundang CHA hospital, CHA University and obtained blood sample. Using DNA extract from white blood cell, we analyzed mir‐196a2, mir‐149, mir‐499 and mir‐146a expressions by PCR‐RFLP and patients’ survival. Results Mean age of patients was 66.9 ± 10.7 yrs old and fifty five was male and thirty nine was female. Median survival was 210 days (18 – 1290 days) after diagnosis of chlangiocarcinoma. According to genetic variant of mir‐196a2, wild type (TT) showed significantly longer survival than in variant type (CT, CC) (P = 0.018), especially in male patients (p=0.011). There was no association between mir‐149mir‐499 and mir‐146a polymorphism and survival of cholangiocarcinoma patients. Conclusion These findings suggest that genetic variants of microRNA 196a2 sequence could be important to predict survival of cholangiocarcinoma patients, but these findings need to be validated in larger prospective study.