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Melatonin prevents muscle atrophy and the alteration of myosin heavy chain in spinal cord injured rats
Author(s) -
Seo Hyeongsun,
Park Kanghui,
Park Sookyoung,
Javaregowda Palaksha Kanive,
Hong Yonggeun
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb668
Subject(s) - melatonin , muscle atrophy , hindlimb , atrophy , endocrinology , medicine , spinal cord , myosin , spinal cord injury , biology , biochemistry , psychiatry
The purpose of this study was to confirm the behavioral features of spinal cord injury (SCI) and to investigate the effects of melatonin on muscle atrophy and shifts in myosin heavy chain (MHC) in an SCI model. SCIs were surgically induced in eight‐week‐old male Sprague‐Dawley (SD) rats that had been randomly divided into three groups: Control, SCI (n=13), and melatonin‐treated after SCI (SCI+MT, n=13). Melatonin (10 mg/kg body weight) was injected subcutaneously twice a day (07:00 and 19:00) for four weeks, after which the body weight and motor function of the rats were evaluated. The expression of muscle atrophy‐related genes in hindlimb muscles, including MAFbx and MuRF1, and MHC, were also analyzed. The body weight and motor functions of the rats were decreased at the acute phase after SCI. The gene expression of MAFbx and MuRF1 were increased in the hindlimb muscles ( p <.05), indicating SCI‐induced muscle atrophy. Melatonin contributed to motor recovery, decreased MAFbx and MuRF1 gene expression, and altered MHC expression in the hindlimb muscles ( p <.05). Thus, melatonin may promote functional recovery, prevent muscle atrophy, and alter MHC expression after SCI. Funding: BioGreen21 Program (Code No. 20070401‐034‐006‐009‐02‐00), Rural Development Administration, Korea