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Macrophage migration inhibitory factor mediates the antidepressant actions of voluntary exercise
Author(s) -
MOON HYO YOUL,
Kim Se Hyun,
Kim Yong Sik,
Ryu Sung Ho,
Suh PannGhill
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb636
Subject(s) - behavioural despair test , antidepressant , neurotrophic factors , macrophage migration inhibitory factor , brain derived neurotrophic factor , proinflammatory cytokine , medicine , neurotransmitter , pharmacology , endocrinology , hippocampus , cytokine , psychology , inflammation , receptor
Voluntary exercise is known to have effects on various brain functions, including an antidepressant action. However, underlying mechanism of exercise effects on psychological disease is still ambiguous. We identified the candidate target genes for alleviating depression by the analysis of existing microarray data sets of mouse hippocampus mRNA from voluntary exercise and ECS (Electroconvulsive seizure) stimulated experiments. To validate the precise changes of those candidate genes, we conducted 28 day voluntary exercise and 10 day chronic ECS treatment with rats. Reverse transcription‐PCR and real‐time PCR analysis showed 5 genes; BDNF, COX‐2, SLC1A1, NPY and MIF were commonly induced by both ECS and voluntary exercise experiments. Furthermore, immunohistochemistry analysis firmly remarked that the amount of MIF, a proinflammatory cytokine, was also increased by voluntary exercise. We showed that recombinant MIF administration produced antidepressant‐like behavior in animal FST (Forced swim test). Moreover, MIF treatment for 1 hour increased the expression of TPH2, a rate‐limiting enzyme in synthesis of serotonin, and 12 hour treatment increased the amount of BDNF in Neuro‐2a neuroblastoma cell line. These results suggest the new role of MIF in relieving the symptom of depression by exercise, probably via enhancing neurotransmitter and neurotrophic factor in the brain.