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Isoleucine administration downregulates the genes for gluconeogenic rate‐limiting enzymes
Author(s) -
Oikawa Nodoka,
Ishii Shunsuke,
Saito Kenji,
Zhao ZhiLei,
Kato Hisanori,
Sugahara Kunio,
Yoshizawa Fumiaki
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb628
Subject(s) - phosphoenolpyruvate carboxykinase , gluconeogenesis , isoleucine , endocrinology , biology , medicine , gene expression , glycolysis , biochemistry , enzyme , chemistry , gene , amino acid , leucine
Isoleucine, a branched chain amino acid, plays an important role in the improvement of glucose metabolism as evidenced by the reduction of gluconeogenesis in liver, along with an increase of glucose uptake in the muscle. This study evaluated the effect of isoleucine on the expression of hepatic gluconeogenic rate‐limiting enzymes in fasted rats. The expressions of phosphoenolpyruvate carboxykinase (PEPCK) mRNA and glucose‐6‐ phosphatase (G6Pase) mRNA in isoleucine‐administered rat liver were decreased compared with that in the fasted rat liver. These ndings imply that isoleucine downregulates the expression of gluconeogenic rate‐limiting enzyme genes and inhibits glucose production in the liver. We also compared the gene expression profiles in the liver of isoleucine‐administered rats and fasted rats by DNA microarray. The gene expression data, although preliminary, indicated that there were changes in the expression of genes for enzymes associated with glycolysis or gluconeogenesis. In addition, pathway analysis using Ingenuity Pathways Analysis software identified biological interaction networks among PEPCK regulated genes.