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Characteristics of early‐stage experimental middle cerebral occlusion‐reperfusion
Author(s) -
Park Sookyoung,
Lee Sangkil,
Chang Kyutae,
Hong Yonggeun
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb610
Subject(s) - medicine , pathogenesis , ischemia , stroke (engine) , middle cerebral artery , pathology , anesthesia , cardiology , mechanical engineering , engineering
Middle cerebral artery occlusion (MCAo)‐reperfusion microsurgery induces focal cerebral ischemia (FCI) and neurological deficiency. Recently, it was reported that integral membrane proteins known as caveolins (cav) may be involved in the pathogenesis and mechanism of cerebral ischemia. Therefore, in this study we established the behavioral features and investigated the expression of cav and signaling molecules in rat brains subjected to MCAo. All rats were evaluated for neurological and motor dysfunction using the modified neurological severity score (mNSS), gait analysis, and beam‐walking test at 24, 48, and 72 h after MCAo. Next, the brains of the rats were cut into 2‐mm thick coronal sections, which were subjected to TTC staining for confirmation of the infarct volume. On assessing the behavior of the MCAo rats, neurological deficiency, motor dysfunction, and impaired balance were detected ( p <.05). Moreover, the gene expression of cav, nNOS, BDNF, and Trk family was decreased, whereas iNOS gene expression was increased in the MCAo group, as compared to the control group, at 24 h after microsurgery ( p <.05). Also, the cerebral infarct volumes were increased significantly in the MCAo group, as compared to the control group ( p <.05). These results imply that cav and signaling molecules, especially iNOS, are a strong causative factor in the early stages of brain damage after FCI. Funding: BioGreen21 Program (Code No. 20070401‐034‐006‐009‐02‐00), Rural Development Administration, Korea