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Beneficial effect of melatonin on improvements of neurological function in focal cerebral ischemia
Author(s) -
Lee Seunghoon,
Lee Sangkil,
Chang Kyutae,
Hong Yonggeun
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb609
Subject(s) - melatonin , saline , neurotrophic factors , medicine , ischemia , endocrinology , neurotrophin , oxidative stress , brain derived neurotrophic factor , inflammation , anesthesia , receptor
Ischemic stroke involves oxidative damage caused by the cellular accumulation of calcium; thus, in the present study, we investigated the effect of neurological dysfunction on various markers related to anti‐oxidant and anti‐inflammatory processes in brain tissue obtained from focal cerebral ischemic rats, and we assessed the influence of long‐term melatonin treatment. Male Sprague‐Dawley rats (n=25, eight‐week‐old) were divided into five experimental groups: group‐A (untreated control), group‐B (vehicle, treated with 0.9% saline for eight weeks), and groups‐C through ‐E (MCAo‐induced focal cerebral ischemic rats treated subcutaneously with melatonin at 10 mg/kg/day at 07:00, 19:00, and 07:00 plus 19:00, respectively). After eight weeks of treatment, the rats were sacrificed by decapitation, and their brains collected for RNA isolation. The differential expression of such inflammation‐ and neurotrophic‐related genes as iNOS , nNOS , and BDNF was then examined. The expression of iNOS and nNOS was decreased in groups‐C and ‐E, as compared to that in the vehicle group, while expression of the neurotrophic factor BDNF was significantly increased in the melatonin‐treated groups. Our results indicate that melatonin decreases the expression of anti‐inflammatory and anti‐oxidant‐related genes. Funding: BioGreen21 Program (Code No. 20070401‐034‐006‐009‐02‐00), Rural Development Administration, Korea