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Effects of Adenovirus AMP activated protein kinase (AMPK) on Scopolamine‐induced Memory Impairment in Rats
Author(s) -
Kim SooJeong,
Lee JunHo,
Liu Jian,
Kim Yong,
Lee Hyo Eun,
Shin MinKyu,
Hong MooChang,
Kim Yangseok,
Bae Hyunsu
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb606
Subject(s) - ampk , morris water navigation task , protein kinase a , neuroprotection , amp activated protein kinase , hippocampus , hippocampal formation , endocrinology , chemistry , medicine , kinase , biochemistry
AMP‐activated protein kinase (AMPK) is an important regulator of energy metabolism. AMPK is activated in cellular stresses such as ischemia, glucose depletion, and exercise, when intracellular levels of AMP increase. In this study, we investigated the neuroprotective effect of AMPK on scopolamine‐induced memory impairment in rats. We constructed a c‐Myc‐tagged AMPK wild type alpha subunit‐encoding adenovirus (Ad‐AMPK‐WT) and a dominant negative adenovirus (N157A, Ad‐AMPK‐DN), which were injected into the hippocampus of Sprague‐Dawley rats with sterotaxic apparatus. The cognition improve effect of AMPK was studied using Morris water maze test in scopolamine‐induced amnesia animal models. The Ad‐AMPK‐WT injected rats significantly improved escape latency of the Morris water maze. Moreover, the activity of acetylcholinesterase was significantly higher in the Ad‐AMPK‐WT injected rats than in the Ad‐AMPK‐DN injected rats. These data demonstrated that AMPK might be useful in the treatment of cognitive dysfunction, and its beneficial effects are mediated via protective effect of hippocampal neuron. This work was supported by the Korea Science and Engeneering Foundation (KOSEF) grant funded by the Korea government (MEST) (no. 2009‐0063466)