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SorLA: a membrane raft protein in glial cells
Author(s) -
Salgado Iris,
Serrano Melissa,
García José,
Martínez Namyr,
Maldonado Hector,
Pagán Carlos Báez,
LasaldeDominicci José A.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb600
Subject(s) - lipid raft , microbiology and biotechnology , lipid microdomain , astrocyte , biology , caveolin , transport protein , cellular compartment , cytoplasm , raft , membrane protein , caveolae , vesicle , membrane , chemistry , cell , biochemistry , neuroscience , signal transduction , polymer , organic chemistry , copolymer , central nervous system
SorLA is a sorting protein in neurons with relevance to Alzheimer's disease (AD). It shares roles with the caveolins, markers of the lipid membrane rafts microdomains. To further our knowledge on sorLA's expression and traffic, we studied sorLA expression in various cultured glia and its relation to caveolin‐1 (cav1), a caveolar microdomain marker. RT‐PCR and immunoblots demonstrated sorLA expression in rat cells: C6 glioma, primary cultures astrocytes (PCRA) and 1321N1 human astrocytoma cells. Differentiation of C6 cells to an astrocyte phenotype (C6 diff) led to a significant decrease in sorLA's mRNA and protein expression. Sucrose density gradient revealed cofractionation of sorLA with cav1 in light density membrane raft fractions of undifferentiated C6, C6 diff and 1321N1 cells. Immunofluorescences analysis of C6 and PCRA revealed a cellular distribution akin to sorLA's staining pattern in neurons. In C6 and PCRA cells a significant percent of sorLA colocalized with cav1, mostly in perinuclear compartments, cytoplasmic vesicles and plasmalemma. These findings establish that sorLA is expressed in glia and is a membrane raft protein involved with cav1 in glial intracellular membrane microdomains trafficking. This study supports a role for glia and its caveolar membrane raft microdomains to the sorting functions of sorLA in the neurodegenerative cascade of AD, unveiling new venues for disease treatment.