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Heterogeneous response of cytokine levels of rat white adipose tissue in cachexia
Author(s) -
Batista Miguel L,
Neves Rodrigo X,
Donatto Felipe F,
Seelaender Marília CL
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb581
Subject(s) - cachexia , adipose tissue , inflammation , white adipose tissue , tumor necrosis factor alpha , medicine , cytokine , endocrinology , proinflammatory cytokine , cancer
The white adipose tissue (WAT) is severely affected by cachexia, a paraneoplastic inflammatory syndrome, in which cytokines, such as TNF‐α and IL‐10 are known to play an important role. Recently, the IL‐10/ TNF‐α ratio has been recently postulated as a marker for the assessment of the degree of inflammation, which correlates with disease‐associated morbidity and mortality. We measured (ELISA) the local concentration of these cytokines in three WAT depots: retroperitoneal (RPAT), epididymal (EAT) and mesenteric (MEAT) of Walker 256 tumour‐bearing rats in a time‐interval of 0 (T0), 7 (T7) and 14 (T14) days after tumour‐cell inoculation (5x10 −5 cells/mL). Higher concentration of TNF‐α was found on T14 of RPAT (2.4‐fold) and of EAT (6.9‐fold) of cachectic rats, both compared with T0. EAT and RPAT IL‐10 levels were reduced on T14 (87.6 and 67.2%, respectively) compared to T0. Regarding the IL‐10/ TNF‐α ratio, there was a decrease in EAT (97.2%) and RPAT (91.4%) after 14 days (T14), compared with T0. The results suggest a heterogeneous local response of both TNF‐α and IL‐10 expression, with a more accentuated reduction in IL‐10 levels of EAT than others depots. We conclude that the degree of inflammation of WAT is dependent of anatomical localization of the pad.