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Isoproterenol‐induced heart failure model in the rat: cardiac autonomic tone, left ventricular function and baroreflex sensitivity
Author(s) -
Dias Daniel Penteado Martins,
Salgado Helio Cesar,
Beltz Terry G,
Xue Baojian,
Johnson Alan Kim
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb561
Subject(s) - baroreflex , propranolol , medicine , ventricle , atropine , cardiology , bradycardia , heart rate , anesthesia , cardiac function curve , basal (medicine) , heart failure , blood pressure , insulin
We investigated the cardiac autonomic tone, left ventricle function and baroreflex sensitivity in rats subjected to isoproterenol‐induced heart failure (HF). Male Sprague Dawley rats received 2 injections (separated by a 24‐hour interval) of isoproterenol (ISO; 170 mg/kg/day, sc; n=8) or vehicle (V; saline; sc; n=8). After 4 weeks, the rats were implanted with femoral artery and vein catheters and tested 3 days later. Cardiac autonomic tone, left ventricle function and baroreflex sensitivity were examined with intravenous injections of atropine (1 mg/kg) and propranolol (2 mg/kg), using Millar catheters and the sequence method. ISO treatment decreased basal HR (ISO 332 vs. V 351 bpm), intrinsic HR (ISO 327 vs. V 347 bpm) and reduced the tachycardia elicited by atropine (ISO 389 vs. V 427 bpm). However, propranolol elicited similar bradycardia in both groups. Rats treated with ISO showed decreased dP/dt+ (ISO 8047 vs. V 10167 mmHg/s) and dP/dt‐ (ISO 8149 vs. V 11428 mmHg/s), but no differences were observed in the baroreflex sensitivity (ISO 1.50 vs. V 1.46 ms/mmHg). Our results extend the characterization of the ISO‐induced HF model and demonstrate a decrease in the basal and intrinsic HR without any change in baroreflex sensitivity.