Premium
Comparison of Human Adipose Derived Stem Cells with Human Bone Marrow Stem Cells in Myocardial Regenerative Therapy
Author(s) -
Srivastava Sapna,
Duong Minh,
Chiu Ray,
ShumTim Dominique
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb544
Subject(s) - stem cell , adipose tissue , bone marrow , cell therapy , bone marrow stem cell , stem cell transplantation for articular cartilage repair , microbiology and biotechnology , adult stem cell , stem cell therapy , cancer research , medicine , pathology , biology , endothelial stem cell , in vitro , biochemistry
Background Cell therapy using Human Bone Marrow Stem Cells (HBMSCs) has been shown to improve heart function after a myocardial infarction. The harvesting technique involved with bone marrow stem cells is invasive and yields a low cell number. There is now an increasing interest in Human Adipose Derived Stem Cells (HADSCs) as they are abundant and readily accessible from liposuction material. The present study was undertaken to investigate if HADSCs are superior than HBMSCs in myocardial regenerative therapy. Results Both HADSCs and HBMSCs proliferated in a time dependent manner, however, the proliferative ability of HADSC was greater than the HBMSCs. In addition, both cells differentiated to the osteoblast lineage confirming their multipotency, when treated with induction medium. Furthermore, treatment of both cells with 5‐AC resulted in positive immunostaining of cardiac markers, troponinI and connexin 43, however the expression of these markers was enhanced in HADSCs. This was further confirmed by western blot analysis, however 5‐AC treatment did not exhibit cell contraction or multinucleation. Conclusion The data suggest that HADSCs may prove to be a more ideal type of cell for regenerative therapy in the future.