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Intraocular transplantation of umbilical cord mesenchymal stem cells rescues retinal functional loss in light‐induced retinopathy
Author(s) -
Lee YihJing,
Lim YahTeem,
Tien LuTai
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb511
Subject(s) - transplantation , retina , retinitis pigmentosa , umbilical cord , retinal , retinal degeneration , stem cell , outer nuclear layer , mesenchymal stem cell , erg , ophthalmology , electroretinography , photoreceptor cell , medicine , biology , anatomy , pathology , surgery , neuroscience , microbiology and biotechnology
Apoptosis of photoreceptor is the final event that leads to degeneration of retina, such as retinitis pigmentosa and age‐related macular degeneration, which often lead to blindness. At present, there are no effective vision‐restoring treatments for such disorders. Stem cell is a potent resource for treating several degenerative diseases including retinal disorders. Among them, the umbilical cord mesenchymal stem cell (UMSC) is a noncontroversial and inexhaustible source for stem cell transplant therapies. The purpose of this study is to investigate the fate of UMSC transplanted into retinas of animal models of light‐induced retinopathy. The rescue effect of UMSC intraocular transplantation was examined functionally by measuring the electroretinogram (ERG). Retinas were harvested for immunohistochemistry and histology analysis by measuring the thickness of the outer nuclear layer (ONL). The data showing 7 days exposure caused significant reductions in a‐wave and b‐wave in ERG. After UMSC transplantation, the amplitude of a‐wave and b‐wave in UMSC‐treated eyes are larger than that measured from non‐treated eyes. The ONL of treated retina was thicker than non‐treated eyes. The transfected UMSCs differentiated into several different kinds of retinal cells. The results indicate that transplantation of UMSCs recovering both function and morphology of retina.