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Effect of Nigella Sativa on Carbon Tetrachloride and Paracetamol Induced hepatotoxicity: Role of Antioxidant Enzymes and Cytokines
Author(s) -
Barakat Mervat kamel,
Oda Naglaa Raafat,
Bayoumy Fatehia Ali,
Bayoumy Fatehia Ali
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb485
Subject(s) - carbon tetrachloride , antioxidant , catalase , nigella sativa , chemistry , superoxide dismutase , glutathione , ccl4 , pharmacology , proinflammatory cytokine , enzyme , biochemistry , traditional medicine , medicine , inflammation , organic chemistry
Objective To investigate the possible protective effect of Nigella Sativa oil (NSO) on carbon tetrachloride (CCL4) and paracetamol (Para) induced hepatotoxicity in view of correlation with antioxidant enzymes and cytokines. Material and Method 40 male albino rats (150–200 gm) were divided equally into 5 groups:group A (control), B (Para treated), C (Para+ NSO treated), D (CCL4 treated), E (CCL4+ NSO treated). Blood samples for estimation of liver enzymes. Liver tissues were used for histopathology study and biochemical assays of antioxidant enzymes Superoxide dismutase (SOD), Catalase (CAT), and Glutathione S‐transferase (GST), and proinflammatory cytokine (IL‐1á). Results The plasma activity of Liver enzymes was significantly increased after treatment with CCL4, and Para. Their level was statistically decreased in rats pretreated with NSO. Tissue activity of GST was significantly higher in group of C as compared to B while SOD and Cat were significantly increased group E as compared to D. IL‐1 á was significantly increased in group E as compared to all groups. Conclusions our results suggest the potential protective role of NSO in CCL4 and Paracetamol induced hepatotoxicity through selective increase of antioxidant enzymes.