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The effect of an experimental model of affective disorder on the expression of GluR2 in rat brain
Author(s) -
Rosa Maria Luiza Nunes Mamede,
Iyomasa Melina Mizusaki
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb465
Subject(s) - hippocampus , entorhinal cortex , hypoactivity , ampa receptor , amygdala , weaning , medicine , endocrinology , schizophrenia (object oriented programming) , nmda receptor , receptor , psychiatry
Glutamatergic hypoactivity may contribute to cognitive dysfunction in schizophrenia. Isolation rearing of rats from weaning has been used as experimental model of affective disorders like schizophrenia. In this study we evaluated the changes in the expression of GluR2 AMPA receptor in hippocampus, amygdala and entorhinal cortex (EC) induced by isolation rearing. In two groups of Wistar rats (n=6/each) the pups remained with their mothers (6/mother) until weaning (21 days) when they were randomly allocated to one of two conditions: 1) grouped, housed 3/cage and handled 3 times/week; 2) isolated, housed individually and handled once/week. After 10 weeks all animals were deeply anaesthetized (50ml/kg of Urethane 25%), perfused and their brains removed. 40‐μm sections were used for immunohistochemistry. The immunopositive cells (IC) were counted bilaterally, in 3 sections/rat, in hippocampus, amygdala and EC. Data were compared by t ‐test ( p <0.05). Isolation rearing induced a significant decrease in GluR2‐IC in hippocampus. This reduction was 52% in the hillus ( p <0.001) and 29% in CA1 ( p =0.002). No change was found in CA3, amygdala or EC. The results suggest that isolation rearing induces changes in GluR2 AMPA receptor in hippocampus similar to those reported for postmortem human brains with schizophrenia, contributing with additional evidence for using isolation of rats from weaning as an animal model of schizophrenia. Grants from FAPESP (05/01501‐7) and FPA.