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Bowel Carcinoma Proliferative Activity in a model of experimental
Author(s) -
Carvalho Sa Pedro,
Cavalcanti Karen,
Coelho Nuno,
Souza Ivone Antonia,
Cabrita Antonio Silverio
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb448
Subject(s) - immunohistochemistry , proliferating cell nuclear antigen , p53 protein , dimethylhydrazine , gastroenterology , adenocarcinoma , chemistry , medicine , pathology , colorectal cancer , cancer
Insecticide 1‐naphtyl N‐methylcarbamate (1NC) has a wide spectrum action and is considered of low toxicity when applied according with the good practice rules. The present work was designed to evaluate morphology and proliferation rates of experimentally induced adenocarcinomas. 30 Wistar 8 weeks old male rats were randomly distributed into three groups to be submitted to dimethylhydrazine (DMH) 15mg/week during 8week. Group II, to 1NC 60mg/l in drinking water during 8weeks. Group III, and without manipulation – Group I. All the animals were sacrificed by the end of the 28th week. Group I rats had no colonic adenocarcinomas while groups II and III exhibited exophytic lesions with wider diameter between 2 and 4 cm. All were well differentiated adenocarcinomas except for one case 1NC induced that was moderately differentiated. Proliferation rate validated by PCNA immunohistochemical study showed to be higher in Group III: 6 negative cases in Group II against 3 negative cases in Group III; the cut off applied + (< 25% positive nuclei), ++ (25 % –75% positive nuclei) and 3+ (> 75% positive nuclei) demonstrated 5 cases of ++ and 3+ in Group III and 1 case 3+ in Group II as the other 3 cases had proliferation rate of +. Despite the small sample explored in this preliminary study, 1NC revealed to induce more aggressive adenocarcinomas validated by higher proliferation rate when compared with the DMH induced cases.