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Bacterial lipoproteins can disseminate from the periphery to inflame the brain
Author(s) -
Londono Diana,
Cadavid Diego
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb416
Subject(s) - inflammation , blood–brain barrier , parenchyma , biology , bacteremia , microbiology and biotechnology , central nervous system , pathology , immunology , medicine , neuroscience , antibiotics
The current view is that bacteria need to enter the brain to cause inflammation. However, in mice infected with Borrelia turicatae we observed widespread cerebral inflammation despite a paucity of spirochetes in the brain parenchyma at times of high bacteremia. Here we studied the possibility that bacterial lipoproteins can disseminate from the periphery across the blood‐brain barrier to inflame the brain. For this we injected normal and infected mice intraperitoneally with lanthanide‐labeled variable outer membrane lipoproteins of B. turicatae and measured their localization in blood, various peripheral organs, and whole and capillary‐depleted brain at various times. Lanthanide‐labeled non‐lipidated lipoproteins and albumin were used as controls. Brain inflammation was measured by TaqMan of genes known to be upregulated in response to borrelial infection. The results showed that the two lipoproteins we studied, LVsp1 and LVsp2, were capable of inflaming the brain after i.p injection to different degree. LVsp1 was better than LVsp2 and Bt1 spirochetes at moving from blood to brain. The dissemination of LVsp1 from the periphery to the brain occurred under normal conditions and significantly increased with infection. In contrast, LVsp2 disseminated better to peripheral organs. We conclude that some bacterial lipoproteins can disseminate from the periphery to inflame the brain.