Premium
Effect of Cold Water Extract from Luffa cylindrical on Intracellular Triglyceride and Free Glycerol Release Level in Lipolysis of Differentiated 3T3‐L1 Adipocytes
Author(s) -
Yang Jiwon,
Jang JaYoung,
Kim OkKyung,
Lee Jeongmin,
Lee Jeongmin,
Jun WooJin,
Hwang KwonTack
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb256
Subject(s) - lipolysis , glycerol , triglyceride , chemistry , lipid droplet , adipocyte , oil red o , 3t3 l1 , intracellular , biochemistry , chromatography , adipose tissue , cholesterol , adipogenesis
The purpose of this study was to investigate the inhibitory effect of Luffa cylindrical cold water freaction(LCC) on intracellular triglyceride and free glycerol release level in lipolysis of differentiated 3T3‐L1 adipocyte. MTT (Methylthiazolyldiphenyl‐tetrazolium bromide) was used to detect the cytotoxic effects of Luffa cylindrical on the viability of 3T3‐L1 cells. Differentiated 3T3‐L1 cells were monitored 80% ethanol, 100% methanol, hot water and cold water fraction from Luffa cylindrical by Oil Red O staining and observed by microscope. Fraction of cold water among them was the most effective in reduction lipid accumulation among four fractions. The treatment of LCC at concentration of 200~1000 ug/mL inhibited the lipid accumulation. These were induced free glycerol release and intracellular triglyceride. These results showed that following treatment LCC was elevated by stimulating lipolysis of differentiated 3T3‐L1 adipocyte. In conclusion, the use of LCC was the most effective in the induction of lipolysis and inhibition of lipid accumulation in 3T3‐L1 cells.