Premium
In vitro digestion/Caco‐2 cell model for assessing transport of dietary folate across the intestinal mucosa
Author(s) -
Yarbaeva Shakhlo N.,
Lee JiYoung,
Albrecht Julie A
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb235
Subject(s) - caco 2 , chemistry , in vitro , legume , incubation , digestion (alchemy) , cell , cell culture , biochemistry , chromatography , biology , botany , genetics
An in vitro digestion coupled to Caco‐2 cell culture model was used to assess and evaluate the permeability of dietary 5‐methyl‐tetrahydrofolate (5‐methyl‐THF) from pinto beans. Folate‐depleted and folate‐sufficient Caco‐2 cell models were used. Caco‐2 cell confluence and integrity of monolayers were validated with transepithelial electrical resistance measurements and red phenol exclusion assay. Samples were 5‐methyl‐THF, 5‐methyl‐THF+2.5% v/v legume extract, 5‐methyl‐THF+5% v/v legume extract, 2.5% and 5% v/v legume extract. Pinto bean extracts at 5% (v/v) were not detrimental to Caco‐2 cells. Permeability coefficients (P app ) were determined in the apical‐to‐basolateral direction. Transport experiments were performed under sink‐conditions. Cumulative fractions of transported 5‐methyl‐THF increased linearly within 80 min of incubation for both folate‐depleted and folate‐sufficient cells. 5‐methyl‐THF was transported across the intestinal mucosa with P app of 1.03–1.79x10 −5 cm/s for samples in folate‐depleted and with P app of 0.99–1.32x10 −5 cm/s for samples in folate‐sufficient cells. This study demonstrates that 5‐methyl‐THF is bioaccessible from bean extracts in Caco‐2 cells. Experimental conditions for use of in vitro digestion/Caco‐2 cell culture model to assess intestinal transport of 5‐methyl‐THF from beans were established.