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Protective effects of Lindera obtusiloba blume treament on tert‐butyl hydroperoxide‐induced oxidative hepatotoxicity and structural analysis of its active compounds
Author(s) -
HONG CHUNGOUI,
KOO YUNCHANG,
NAM MIHYUN,
KIM JIHOON,
WANG ZENG,
YANG SUNGYONG,
LEE KWANGWON
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb217
Subject(s) - quercitrin , chemistry , dpph , antioxidant , ascorbic acid , oxidative stress , traditional medicine , quercetin , food science , ec50 , biochemistry , pharmacology , medicine , in vitro
The Lindera obtusiloba blume (LO), a native plant in East Asia, trdionally used as a popular folk. The aim of this study was to evaluate the protective effects of LO extract (LOE) on the tert‐butyl hydroperoxide (t‐BHP)‐induced oxidative hepatotoxicity and confirmed the structure of its active compound with NMR. The study of liver protection showed that pretreatment with LOE (500 or 2000 mg/kg) by gabage to SD rats for 7 days before a single dose of t‐BHP (0.2 mmole/kg i.p.) significantly lowered the serum levels of the hepatic enzyme markers, aspartate aminotransferase and alanine aminotransferase and reduced the indicators of oxidative stress in the liver, such as lipid peroxidation. The active compounds were identified as quercetin 3‐O‐¥á‐rhamnoside (quercitrin) and kaempferol 3‐O‐¥á‐rhamnoside (afzelin). In antioxidant effects of quercitrin and afzelin, ferric‐reducing antioxidant power (FRAP) value were 3.05 ¡¾ 0.42 and 0.77 ¡¾ 0.04 mmol FeSO4․7H2O/§· DM, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) EC50 value were 107.45 ¡¾ 4.12 and 438.71 ¡¾ 14.18 ¥ìM. FRAP value of quercitrin was 1.5‐fold higher than ascorbic acid (1.90 ¡¾ 0.26) which is a powerful antioxidant and also DPPH EC50 value of quercitrin was 2‐fold higher than ascorbic acid (192.90 ¡¾ 10.78). Thus, it has been suggested that these compounds might contribute to the health positive effects of LO against t‐BHP induced hepatotoxicity.