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Signalling pathway of prostate‐specific membrane antigen implicates different types of detergent‐resistant membranes
Author(s) -
Schmidt Sonja,
Heine Martin,
Naim Hassan Y.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb179
Subject(s) - glutamate carboxypeptidase ii , internalization , prostate cancer , membrane , chemistry , antibody , cell , antigen , prostate , cell membrane , microbiology and biotechnology , mapk/erk pathway , signal transduction , cancer research , cancer , biochemistry , biology , medicine , immunology
Prostate‐specific membrane antigen (PSMA) is a surface protein overexpressed in prostate cancer and neovasculature of further tumours. It is therefore one of the most promising biomarkers in diagnosis and treatment of prostate cancer. PSMA is associated with detergent‐resistant membranes (DRMs). The mature form of PSMA is mainly insoluble in Lubrol WX, but does not associate with Triton X‐100 DRMs. Recently we could demonstrate that cross‐linking of cell surface PSMA with specific antibodies activates different signalling cascades like the MAPK‐pathway. Here we show that internalization of PSMA is increased after antibody binding and this leads to redistribution of PSMA to Triton X‐100 DRMs in a time‐dependent manner. Our results propose an essential role for different DRM types in the signalling pathways involving PSMA.