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Effect of Advanced Glycation Endproducts on Human Smooth Muscle Cell proliferation by Co‐culture System with Endothelium and Monocytes.
Author(s) -
Nam MiHyun,
Lee HyunSun,
Hong ChungOui,
Koo YunChang,
Kim Ji Hoon,
Wang Zeng,
Yang SungYong,
Lee Yan Houy,
Lee KwangWon
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb138
Subject(s) - umbilical vein , endothelium , monocyte , fibronectin , cell culture , cell growth , chemistry , immunology , microbiology and biotechnology , medicine , biology , in vitro , extracellular matrix , biochemistry , genetics
Atherosclerosis is one of the consequence of long‐term diabetic complications due to the accumulation of lipids and fibrosis in the artery wall. Advanced glycation endproducts (AGEs) may play an important role in the pathogenesis of diabetic complications. Atherosclerosis initiates adhesion of monocytes which induced by AGEs to the endothelium and transmigration across tight‐juction of endothelial cells (EC) into intima. Monocytes differentiated into macrophages which uptook lipoproteins and became foam cells. Smooth muscle cells (SMC) were then migrated from media to surface and secreted various fibrous elements. The aim of this study is to confirm the proliferation of SMC by AGE in a co‐culture system in which monocyte, EC and SMC were employed. THP‐1, human acute monocytic leukemia cells and HUVEC, human umbilical vein endothelial cells were cultured for 48 hours on the 2% gelatin coated upper chamber and HA‐VSMC, human artery vascular smooth muscle cells in the lower chamber. In a group treated with AGEs, SMC were co‐cultured with HUVEC and THP‐1 with 100 μg/mL of AGEs for 48 hours. Proliferation of SMC was measured by MTT assay. We observed proliferation of SMC by AGEs that using our co‐culture system. Moreover, AGEs treated SMC also induced several fibroblast markers on mRNA levels including fibronectin, collagen type 3 by this co‐culture system.