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Effect of Methylglyoxal on BV‐2 Cells
Author(s) -
Gorgis Tanya,
Habib Nawaf,
Mungre Sue
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.lb132
Subject(s) - methylglyoxal , nitric oxide , oxidative stress , chemistry , inflammation , macrophage , antioxidant , viability assay , diabetes mellitus , biochemistry , pharmacology , microbiology and biotechnology , cell , immunology , endocrinology , in vitro , biology , enzyme , organic chemistry
Inflammation is believed to play a role in complications seen in diabetic patients. Methylglyoxal (MG) a normal molecule in the cell is believed to increase under diabetic conditions. MG may activate macrophages that release pro‐inflammatory cytokines. We used BV‐2 cells to determine the effect of MG on the viability and activation of macrophages. Our results show that MG causes proliferation of BV‐2 cells. There is increased production of nitric oxide suggesting that cells are activated. Curcumin, an antioxidant reduced nitric oxide production by about 30%. These results suggest that activated macrophage cause inflammatory conditions in diabetes due to oxidative stress and antioxidant have a protective effect