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Changes in oxidative modifications of skeletal muscle proteins in old rats fed a high fat diet
Author(s) -
MURAKAMI Hitoshi,
GUILLET Christelle,
TARDIF Nicolas,
SALLES Jerome,
BOIRIE Yves,
WALRAND Stephane
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.997.12
Subject(s) - medicine , endocrinology , nitrotyrosine , myofibril , insulin resistance , sarcopenia , mitochondrion , skeletal muscle , oxidative phosphorylation , western blot , oxidative stress , chemistry , biology , insulin , biochemistry , nitric oxide synthase , nitric oxide , gene
Muscle weight loss during aging results from change in protein turnover, but oxidative modifications of proteins also occur in muscle. In addition, dietary fat promote accumulation of modified proteins in tissues. Therefore, high fat diet in old rats may be associated with change in protein turnover and modifications, contributing to sarcopenia. To elucidate this hypothesis, nitrotyrosine (NT) in quadriceps was measured by Western‐Blot in 6 months and 24 months old rats fed a normal fat (NF) or a high fat diet (HF) for 3 months (adult‐NF (n=7), adult‐HF (n=8), old‐NF (n=7), old‐HF (n=7)). NT was significantly increased in old rats, although there was no effect of dietary fat on NT accumulation. However, in some specific mitochondria proteins (100kDa), NT increase was dependent on aging and dietary fat. Interestingly, NT was strongly negatively correlated with muscle mass ( r 2 =−0.716 (p<0.0001), −0.497 (p<0.01) and −0.699 (p<0.0001)) in sarcoplasma, myofibril and mitochondria, respectively). In addition, NT in specific mitochondria proteins was positively correlated with insulin‐resistance evaluated by OGTT ( r 2 =0.535 (p<0.05)). Therefore, NT in muscle proteins is associated with muscle weight loss during aging but not in HF. In addition, NT in specific mitochondria proteins is associated with insulin resistance induced by a high fat diet.