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Low salt intake during pregnancy and lactation causes changes in energy metabolism in adult female Wistar offspring
Author(s) -
Jang Karen Lucasechi Lopes,
Furukawa Luzia Naoko Shinohara,
Dolnikoff Miriam Stermann,
Heimann Joel Claudio
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.991.5
Subject(s) - offspring , endocrinology , medicine , weaning , lactation , thermogenin , leptin , brown adipose tissue , metabolism , pregnancy , thermogenesis , chemistry , adipose tissue , biology , obesity , genetics
Objective To study some factors related with the energy metabolism in adult offspring of dams fed low salt diet during pregnancy and lactation. Methods Female Wistar rats were fed low (LS 0.15%) or normal (NS 1.3% NaCl) salt diet since 8 weeks of age. At 12 weeks of age, they were mated. Offspring were fed NS from weaning and at 12 weeks of age, they were kept on 4 o C or room temperature (RT) during 24 hours. Body weight (BW) and temperature (BT), blood glucose (GLU) plasma norepinephrine (NOR), and leptin (LEP) were measured. Gene expression (RT‐PCR) for uncoupling protein‐1 (UCP1), coenzyme Q1 (Q1) and Q2 (Q2) was evaluated in brown adipose tissue. Results (mean±SEM, n=5 to 8/group): No differences were observed in BW between adult offspring groups. UCP1, Q1 and Q2 were lower (p<0.05) in LS (UCP1 ‐ 1.5±0.1, Q1‐ 0.7±0.1, Q2 ‐ 1.0±0.1) than in NS (UCP1 ‐ 2.0±0.1, Q1 ‐ 1.1±0.1, Q2 ‐ 1.3±0.1) at RT but not at 4°C. At 4°C BT decreased (p<0.05) in NS but not in LS. NOR (pg/dL) was higher on 4°C (471±42) than on RT (206±28) in NS but not in LS. GLU did not change after exposure to 4°C in NS and LS. At 4°C, LEP decreased (p<0.05) in NS but not in LS. Conclusions Low salt diet during pregnancy and lactation modifies expression of some genes involved in energy metabolism in adult female offspring. Supported by FAPESP.