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Effect of stratum corneum hydration on the composition of sweat collected by an occlusive sweat patch
Author(s) -
Mack Gary W.,
Taylor Penny
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.991.24
Subject(s) - sweat , stratum corneum , chemistry , iontophoresis , distilled water , medicine , chromatography , pathology , radiology
We tested the hypothesis that sweat trapped on the skin with an occlusive sweat patch will leach electrolytes from the stratum cornea (SC) and alter sweat composition. 20 subjects completed 40 minutes of moderate exercise in the heat and select skin sites were hydrated before exercise by adhering cylinders of distilled water to the skin. SC samples were taken before and after exercise using a tape stripping method and sweat samples were collected with an occlusive sweat patch. SC hydration was verified by a reduction in [K + ] SC (p < 0.05). SC hydration decreased [K + ] sweat (8.14 ± 0.46 to 6.56 ± 0.46 mmol/l,) and [lactate] sweat (11.64 ± 1.36 to 8.82 ± 1.11 mmol/l) at hyperhydrated skin sites (p < 0.05). Without an occlusive sweat patch [Na + ] SC and [K + ] SC increased after sweating (p < 0.05) but not with an occlusive sweat patch. Our data indicate that during normal sweating the SC “dehydrates” resulting in an increase in the SC electrolyte concentration. An occlusive dressing traps sweat on the skin and prevents water movement out of the SC (rather than electrolyte leaching) and thereby produces a more concentrated sweat.