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Elevated IL‐1RA levels blunt exercise‐induced increases in Hsp25 and Hsp72 in mouse muscle
Author(s) -
Huey Kimberly,
Meador Benjamin,
Freund Gregory
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.989.9
Subject(s) - heat shock protein , endocrinology , medicine , western blot , saline , antagonist , receptor antagonist , genetically modified mouse , receptor , insulin resistance , shock (circulatory) , transgene , biology , insulin , gene , biochemistry
Evidence using heat or transgenic up‐regulation of heat shock proteins (Hsp)72 and 25 suggest they may protect against obesity‐induced insulin resistance. We reported that running wheel training significantly increased Hsp72 and Hsp25 in active muscles of lean mice suggesting their contribution to improved glucose regulation with exercise. However, obesity is associated with higher levels of circulating IL‐1 receptor antagonist (IL‐1RA) that may impact Hsp up‐regulation. We tested the hypothesis that high IL‐1RA levels blunt exercise‐induced Hsp up‐regulation in muscle by blocking the actions of IL‐1. We compared Hsp72 and Hsp25 protein levels with Western blot in muscle of C57 mice (n=4/group) following 3 days of wheel running ± Kineret® (KIN), a recombinant IL‐1RA. Exercise increased Hsp25 and Hsp 72 protein from 0.08±0.02 to 0.16±0.03 and 0.10±0.04 to 0.19±0.03 arbitrary units in saline groups, respectively. No changes in either Hsp were seen with exercise and KIN. Average daily wheel running time (4.6±0.5 vs. 4.6±0.4 h) and distance (4.0±0.5 vs. 4.1±0.4 km) was not different between saline and KIN, respectively. These results suggest that high levels of circulating IL‐1RA may attenuate Hsp up‐regulation in active muscles. However, longer‐term training may reduce adiposity which subsequently may decrease circulating IL‐1RA and improve Hsp responses. Funded in part by NIH DK64862 and NS58525 to GGF