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Influence of myostatin on skeletal muscle plasticity
Author(s) -
ReiszPorszasz Suzanne,
Abraham Andrea,
Baker Michael,
Kovanecz Istvan,
Porszasz Janos,
Caiozzo Vincent J.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.989.17
Subject(s) - plantaris muscle , myostatin , skeletal muscle , medicine , endocrinology , biology , transgene , genetically modified mouse , muscle hypertrophy , anatomy , gene , biochemistry , soleus muscle
The objective of this study was to investigate myostatin (Mst) role in the process of muscle structural and functional changes in adult skeletal muscle. Myostatin (growth and differentiation factor‐8, GDF‐8) regulates skeletal muscle growth and differentiation during embryogenesis, but its function in adult muscle is not understood. Three genetically altered animal (6 month old) models were used for bilateral compensatory overload experiment: Mst‐null (KO) mice, Mst overexpressing transgenic (TG) and conditional Mst overexpressing transgenic (CMOT, first time reported) compared with wild type (WT) males (five animals/group). Plantaris muscles were analyzed for contractile measurement, morphometry, histochemistry and gene expression profile. Plantaris muscle weight increased in all groups, but biggest increase was found in CMOT (200%) and the smallest was in KO (158%) mice. The same tendency was observed in total plantaris muscle cross sectional area changes. Fiber transition from oxidative to glycolytic fiber was identified in each group with the highest rate in KO (17%). Fiber number increased after overload indicating that Mst does not inhibits cell proliferation in adult muscle. We conclude that Mst plays a different role in muscle during adulthood regulating muscle structure, composition, and physical performance. This study was supported by 1R21AR054101, G12RR030262, and P20MD000545 grants.