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Characterization of LPA4 and LPA5 In zebrafish
Author(s) -
Kao Milky ShengYung,
Chang ChigHan,
Lee HsinYu
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.988.9
Subject(s) - zebrafish , receptor , lysophosphatidic acid , biology , microbiology and biotechnology , morphogenesis , g protein coupled receptor , signal transduction , gene , genetics
Lysophosphatidic acid (LPA) is a simple lysophospholipid which regulates various important biological functions such as cell proliferation, migration, differentiation, and morphogenesis. Multiple receptors for LPA on cell membrane have been identified, designated LPA1‐7. Among these receptors, LPA1‐3 are the members of the endothelial differentiation gene (EDG) family. In contrast, LPA4‐5 are first discovered as purinergic receptors in P2Y family. In zebrafish (Danio rerio), LPA1 and LPA3 were demonstrated to be important regulators for lymphatic vessel and neuronal development, while the roles of other LPA receptors have not yet been identified. As we search LPA receptors by TBLASTN in zebrafish nucleotide database, we found LPA4 and one sequence similar to both mouse LPA5 and P2Y5. After alignment with all known LPA receptors, the sequence shows more similarity with LPA5 than with P2Y5. Our observation suggest that LPA5 gene evolutionally multiplied and differentiate into two receptors. In zebrafish, zLPA4 expressed in gill, testis and spleen, while zLPA5 expressed in most tissues after 18‐somite stage. However, LPA treatment was unable to induce Ca++ mobilization in zLPA4 or zLPA5 transfected B103 rat neuroblastoma, indicating that zebrafish LPA receptors might not trigger conventional signaling cascades of mammalian LPA receptors. Institute of Zoology, National Taiwan Universtiy