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Tensorial Description of the geometrical arrangement of Endothelin‐1 in response to the changes of blood pressure in rat's aorta
Author(s) -
Huang Wei,
Mak Yi Wah
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.988.2
Subject(s) - aorta , abdominal aorta , anatomy , blood pressure , blood vessel , thoracic aorta , chemistry , medicine
For better studying the vascular remodeling in hypertension, the geometrical arrangement of the cellular molecules, which are the foundation of the physical behavior of the cells and tissues, has to be described quantitatively. Endothelin‐1 (ET‐1) is a vasoconstrictor and mitogenic agent, and has been found in the vascular endothelium, the vascular smooth muscle cells, and the fibroblasts. This study aimed to investigate quantitatively the changes of the geometrical distribution of ET‐1 in blood vessel wall in response to the changes of blood pressure. By clamping one cross section of the rat's abdominal aorta, the blood pressure in the aorta was raised in the portion above the clamp, and lowered in the portion below the clamp. The effects of the raising or the lowering of the blood pressure on these two portions of the aorta simultaneously on the tissue remodeling in the abdominal aortas were studied after aortic banding for 0, 0.5, 4, or 8 days. Aortic cross sections in circumferential and longitudinal directions and En Face were prepared and immunostained with ET‐1 antibody for examination by a fluorescent microscope. Digital images were taken for analysis. The geometrical distributions of ET‐1 in the vessel wall was described by a Molecular configuration tensor, P ij , where the subscripts i, j refer to the Cartesian coordinate axes x 1 , x 2 , x 3 . All components in the molecular configuration tensor of ET‐1 are presented.