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The Effect Of Ethanol On Tracheal Smooth Muscle Of Cat
Author(s) -
Jakupaj Muharrem,
Sopi Ramadan B,
Lajçi Azem,
Neziri Burim,
DevajaKumnova Shqipe,
Krasniqi Avdyl
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.986.6
Subject(s) - chemistry , ethanol , contraction (grammar) , nifedipine , nitric oxide synthase , nitric oxide , biophysics , channel blocker , muscle contraction , incubation , medicine , endocrinology , pharmacology , biochemistry , calcium , biology , organic chemistry
Previously we have shown that ethanol induces contraction of pulmonary artery smooth muscle (PASM). However, the effect of ethanol on tracheal smooth muscle (TSM) and the mechanisms involved are not well understood. Therefore, we hypothesized that ethanol induces contractile responses of TSM and extra cellular Ca 2+ is involved to this effect. In vitro force measurements were performed in TSM strips, excised from cats (n=29). Tissues were stabilized in Krebs‐Henseleit (KH) solution and treated with different concentrations of ethanol (46 – 276 mM). The role of extra cellular Ca 2+ was studied by incubation of tissue in a Ca 2+ free KH solution or using a Ca 2+ channel blocker‐nifedipine. The role of nitric oxide (NO) was assessed by inhibition of NO synthase (NOS) using N ω ‐nitro‐L‐arginine methyl ester. The ethanol induced concentration‐dependent contraction of TSM. Lack of Ca 2+ in medium resulted with decrease of TSM tone likewise Ca 2+ channel blocker. Inhibition of NOS, significantly increased contractile responses of TSM to the ethanol at lower ad middle concentrations, but reduced these responses at higher concentrations. In addition, inhibition of NOS significantly potentiated TSM tone to ACh. These data demonstrate that ethanol induces TSM contraction of cat and extra cellular Ca 2+ is an important involved factor. NO release as a relaxant molecule counteracts the effect of ethanol and other agonists.