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Alteration of guanylyl cyclase‐activated vasorelaxation in thoracic aorta from hyperthyroid rats
Author(s) -
Wen Jin Fu,
Jin Song Nan,
Wang Yan Li,
Zhou Guang Hai,
Wang Xin g,
Cho Kyung Woo
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.986.5
Subject(s) - soluble guanylyl cyclase , euthyroid , nitric oxide , thoracic aorta , aorta , guanylate cyclase , medicine , endocrinology , natriuretic peptide , chemistry , thyroid , heart failure
Objective Nitric oxide (NO) and C‐type natriuretic peptide (CNP) activate soluble (sGC) and particulate guanylyl cyclas e (pGC), respectively, and play important roles in the mainte nance of cardiovascular homeostasis. The purpose of the present study was to define the alteration of pGC‐ and sGC‐ac tivated vasorelaxation in the thoracic aorta from hyperthyroid rats. Methods and Results Hyperthyroidism was induced by administering L‐thyroxine (T4, 0.05 mg/100 g, sc) daily for 16 days. Experiments were performed in isolated rat aortic rings. CNP (0.1–1000 nM) and SNAP (10‐10000 nM) induced vascular relaxation in a concentration‐dependent manner in aortic rings from eu‐and hyperthyroid rats. The responses were significantly attenuated in aortic rings from hyperthyroid rats. ODQ (10 uM), an inhibitor of sGC, blocked the SNAP ‐induced vasorelaxation of aortic rings from both eu‐ and hyperthyroid d rat. To define the changes in response to cGMP, 8‐Br‐Cgmp (1–100 uM), a cell‐permeable cGMP analog, was used. 8‐Br‐cGMP‐induced vasorelaxation was attenuated in aortic rings from hyperthyroid rats compared to those from euthyroid rats. Conclusions These data demonstrate that pGC‐ and sGC‐activated vaso relaxation is attenuated in thoracic aorta from hyperthyroid rats which may be a part of the pathophysiology of the disease. Supported by NSFC (No30570669; 30971080).