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Heterogeneity in Constrictor Responses Among Selected Inbred Mouse Strains: Influence of Rho‐kinase
Author(s) -
Didion Sean P
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.986.13
Subject(s) - rho associated protein kinase , contraction (grammar) , medicine , phenylephrine , endocrinology , rho kinase inhibitor , kinase , serotonin , myosin light chain kinase , chemistry , biology , myosin , biochemistry , receptor , blood pressure
The goal of this study was to compare and contrast contractile responses among four commonly used inbred mouse strains and to determine the contribution of Rho‐kinase. Contractile responses were examined in carotid arteries (in vitro) from C57BL/6, C3H/He, Balb/c, and SWR mice. We examined constrictor responses to potassium chloride (KCl; 1–100 mM), serotonin (5HT; 1 nM–10 μM), phenylephrine (PE; 1 nM–10 μM), and U46619 (0.03 – 3 mg/ml). 5HT produced a significantly greater degree of contraction in Balb/c mice compared to C57BL/6, SWR and C3H/He mice (eg, 100 μM 5HT produced 0.45±0.06, 0.14±0.03, 0.13±0.02, and 0.08±0.03 mg of contraction in Balb/c, C57Bl/6, SWR, and C3H/He mice, respectively). Contraction to PE and U46619 was also markedly greatest in Balb/c mice. KCl responses were similar in all strains. In order to examine the contribution of Rho‐kinase, carotid arteries were incubated with either vehicle or Y‐27632 (1 μM; Rho‐kinase inhibitor). Y‐27632 reduced contractile function in a constrictor‐ and strain‐dependent manner (eg, contraction to 100 μM 5HT with Y27632 = 0.22±0.04, 0.06±0.01, 0.04±0.01, and 0.03±0.01 mg of force in Balb/c, C57Bl/6, SWR, and C3H/He mice, respectively). These data provide evidence for heterogeneity in constrictor responses among four inbred mouse strains and dependency upon Rho‐kinase.