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Monoamine oxidase activity in skeletal muscle conduit and resistance vessels
Author(s) -
Evanson Kirk W,
Stone Audrey J,
Kluess Heidi A
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.985.6
Subject(s) - monoamine oxidase , anatomy , chemistry , biology , enzyme , biochemistry
The purpose of this study was to examine monoamine oxidase (MAO) activity in the vascular smooth muscle of conduit and resistance vessels. Femoral arteries and red (RG1A) and white (WG1A) first‐order gastrocnemius arterioles were excised from 6‐month old male, F344 rats (n=5). The vessels were cut into 2mm segments and homogenized in 120μl of enzyme buffer (100mM HEPES, 5% glycerol). Luminometric (Promega) and spectrophotometric (Thermo Scientific) assays were used to quantify MAO activity and total protein content of the vessel, respectively. Relative luminescence units (RLU) were proportionate to MAO activity. MAO activity was greater in the femoral arteries (18.59±2.26 RLU/μg; p<.05) as compared to RG1A (11.62±1.82 RLU/μg) and WG1A (12.03±1.39 RLU/μg) vessels. These data suggest that MAO activity is greater in the larger conduit vessels than in the resistance vessels irrespective of vessel size, and that there are no differences in MAO activity between the red and white first‐order gastrocnemius arterioles. This research was supported by the Arkansas Biosciences Institute and the National Institute on Aging.