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Endothelin‐1 induces smooth muscle cell plasticity in small mesenteric arteries
Author(s) -
Schoorl Marieke,
Bakker Erik N,
Vos Judith,
vanBavel Ed
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.985.17
Subject(s) - myograph , distension , contraction (grammar) , incubation , chemistry , anatomy , medicine , biochemistry
Aim Small arteries show eutrophic inward remodeling in response to many physiological and pathological stimuli. We hypothesized that early events include repositioning and adapted architecture of smooth muscle cells, reflected by shifts in active length‐tension relations. Methods Rat mesenteric arteries were incubated overnight in a wire myograph at low (0.4 D100) or high (1.1 D100) distension with or without 10 nM endothelin‐1 (ET‐1). D100 is the passive diameter at an equivalent pressure of 100 mmHg. Before and after incubation, active (125 mM K + ) and passive force‐length relationships were determined by stepwise distension of the vessel. Results Segments (n=10) incubated with ET‐1 at 0.4 D100 showed a marked leftward shift of the force‐length curve. Optimal force developed at 0.90±0.02 D100 (day 0) versus 0.84±0.02 D100 (day 1, p=0.03). This shift did not occur in the absence of ET‐1. At 1.1 D100 incubation, slight rightward shifts occurred. Conclusion maintained activation at low diameter shifts tension‐generating properties, possibly forming an early event in inward remodeling. This study was performed within the framework of the Dutch Top Institute Pharma project T2‐108.