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Age‐ and sex‐related changes in purinergic metabolism in the skeletal muscle vasculature
Author(s) -
Stone Audrey J,
Evanson Kirk W,
Kluess Heidi A
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.985.10
Subject(s) - medicine , endocrinology , atpase , phosphate , skeletal muscle , chemistry , biology , enzyme , biochemistry
The purpose of this study was to determine if vascular ecto‐ATPase was affected by sex or age in skeletal muscle arteries and arterioles. We hypothesized that females would have higher ATPase activity than males, and that old rats would have lower ATPase activity than young rats. The red gastrocnemius 1A (G1A) arterioles and femoral arteries were removed and cleaned from 2mo, 6mo, and 24mo old Fisher 344 rats. Whole vessel segments were incubated in a phosphate reagent for 50min and then analyzed using a spectrophotometer. Males had significantly higher ATPase activity than females at 2mo (106±22, n=8 vs. 39±7pM phosphate, n=8) and 6mo (208±56, n=7 vs. 44±14 pM phosphate, n=8) in the G1A, p<0.05. Endothelial removal in 2mo G1A reduced the variability (males: 86±7, n=5; females: 71±10, n=5 vs. males: 88±18, n=8; females: 60±17 pM phosphate, n=7), but it was not statistically different. Males (1184±175 pM phosphate) had significantly higher ATPase activity than females (489±36 pM phosphate) in the femoral in 6mo and 24mo (1510±70 vs. 908±76 pM phosphate) rats, p<0.05. In the femoral, ATPase activity was 47% higher in 24mo males than in 2mo old males ( p<0.05). ATPase activity was 50% higher in 24mo females than in 2mo and 6mo females(p<0.05). In general, males appear to have higher ATPase activity than females, and ATPase activity increases with age. Supported by NIH and Arkansas Biosciences Institute.