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The nitric oxide (NO) donors [Ru(terpy)(bdq)NO+]3+ (TERPY) and Sodium Nitroprusside (SNP) have different cellular effects in rat basilar arteries relaxation.
Author(s) -
Paulo Michele,
Vercesi Juliana Aparecida,
Biazzotto Juliana C.,
Da Silva Roberto Santana,
Bendhack Lusiane Maria
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.984.3
Subject(s) - sodium nitroprusside , basilar artery , nitric oxide , soluble guanylyl cyclase , chemistry , potency , relaxation (psychology) , medicine , guanylate cyclase , endocrinology , in vitro , biochemistry
This study aimed to verify if the new NO donor (Terpy) induces vasorelaxation in basilar artery from renal hypertensive (2K‐1C) and normotensive (2K) rats and compare this effect with SNP. We also investigated the effects of inhibition of guanylyl‐cyclase with ODQ (1μmol/L) and K + channels with TEA (1mmol/L). We have analyzed the efficacy (ME) and potency (pD 2 ). The experiments were performed on denuded rings which were pre‐contracted with endothelin‐1 after pre‐incubation with ODQ or TEA. Interestingly, Terpy (1nmol/L to 100mmol/L) did not induce relaxation. On the other hand, SNP induced relaxation in a concentration‐dependent way, which was greater in basilar arteries from normotensive 2K (ME: 91.5±2.5%; pD 2: 5.96±0.19, n=6) than in basilar rings from 2K‐1C (ME: 77.8±3.8%; pD 2 : 4.63±0.15, n=6, P<0.05). TEA had a greater inhibitory effect in 2K arterial rings (ME: 42.0 ± 1.2%, pD 2 5.12 ± 0.25, n=6) than in 2K‐1C arteries in which reduced only the maximal relaxation (ME: 19.4 ± 0.8%, n=6) without effect on the potency of the NO donor. ODQ abolished the relaxation induced by SNP in 2K and 2K‐1C arteries. In conclusion, Terpy did not induce relaxation in basilar artery from 2K and 2K‐1C rats and the relaxation induced by SNP involves the activation of soluble guanylyl‐cyclase sensitive to ODQ and K + channels sensitive to TEA. Supported by FAPESP and CNPq.