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Macrophage Nitrite Uptake and Release
Author(s) -
Kaimootil Sunil,
Newell Clayton,
Tran Tuan,
Rodriguez Juan
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.984.18
Subject(s) - nitrite , extracellular , nitric oxide , intracellular , chemistry , macrophage , biochemistry , cell culture , biophysics , nitrate , biology , in vitro , organic chemistry , genetics
Activated macrophages are known to produce large concentrations of nitrite from the synthesis of nitric oxide. With the recent discovery that nitrite is a biologically active substance, one question that gains more significance is how macrophages hold the nitrite they produce. In this respect, the question of how quickly macrophages internalize or externalize the anion becomes of relevance. Here we report on experiments that characterize the kinetics of nitrite uptake into, and release from the murine J774 macrophage cell line. Cells were exposed to 100 μM of 15 N‐labeled nitrite and intracellular or extracellular concentrations measured as a function of time using a mass spectrometric method developed in our lab. Our results show that transport of the nitrite in and out of the cells is on the order of a minute of less, and that the ensuing intracellular and extracellular concentrations of the anion are similar. The results suggest that macrophage nitrite uptake and release is dominated by passive transport.