Premium
The effect of renal perfusion pressure on renal injury in hyperglycemic AngII hypertensive rats
Author(s) -
Jin Chunhua,
Polichnowski Aaron,
Ohsaki Yusuke,
Cowley Allen W.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.983.5
Subject(s) - kidney , medicine , endocrinology , diabetic nephropathy , diabetes mellitus , blood pressure , excretion , perfusion , streptozotocin , renal function , urology
Hypertension and diabetes often co‐exist and are the leading causes of end stage renal disease. The contribution of renal perfusion pressure (RPP) to diabetic nephropathy was explored in the present study by comparing the left kidney, with RPP chronically servocontrolled (SC), to the right uncontrolled (UC) kidney. Diabetes was induced with a single i.p. injection of streptozotocin (STZ; 65 mg/kg) in male Sprague Dawley rats at 7 wks of age. At 12 wks of age, rats (n=4) were surgically prepared and hypertension produced by i.v. AngII (25 ng/kg/min) + 4% NaCl diet for 14 days then anesthetized for bilateral ureteral urine collection. Over the 14 days of AngII infusion, RPP averaged 173±7 mmHg to the UC kidney and 112±3 mmHg to the SC kidney with plasma glucose 353±45 mg/dL; cholesterol 76±6 mg/dL; triglycerides 146±11 mg/dL. Urinary albumin and protein excretion were significantly greater in the UC kidneys compared to SC kidneys (61.6±14 vs 7.9±5 ug/min; 200±50 vs 49±20 ug/min p <0.05). In a group of non‐STZ AngII + 4% NaCl diet treated rats which reached the same levels of hypertension, albumin excretion averaged 15.4±1.7 ug/min from the UC kidney vs 5.3±0.9 ug/min from the SC kidney. Together, these data indicate that hyperglycemia significantly enhances the susceptibility of the kidney to pressure‐induced injury. (HL‐081091)