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The Isolation of the Receptor for Advanced Glycation End‐Products from Avian Vasculature
Author(s) -
Cousens Emily N.,
Braun Eldon J.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.981.10
Subject(s) - rage (emotion) , glycation , receptor , medicine , polyclonal antibodies , endocrinology , western blot , diabetes mellitus , advanced glycation end product , immunohistochemistry , chemistry , biology , antibody , immunology , biochemistry , neuroscience , gene
Accumulation of glucose in the plasma results in the formation of Advanced Glycation End Products (AGEs) by a non‐enzymatic reaction with albumin. These products bind to Receptors for AGEs (RAGE) on endothelial cells. This alters the basement membrane structure leading to accumulation of fluid in tissues as seen in Type II Diabetes. Humans normally maintain plasma glucose concentrations of 4.4 to 5.5mM/L whereas birds maintain concentrations of 16.6mM/L or greater. If plasma glucose concentrations in humans exceed 6.6mM/L for 4–6 weeks, symptoms of diabetes will begin to develop. Birds can serve as an animal model for the study of Type II diabetes due to their high plasma glucose levels and that they are insulin insensitive resisting the effects of these levels. In the present study, SDS PAGE and Western Blot analysis were used to isolate RAGE from the vasculature of Mourning Doves. RAGE N‐16 goat polyclonal IgG (Santa Cruz) was used as the primary anti‐body which detects the presence of RAGE in mice, rats, humans and cows increasing the likelihood of detecting RAGE in birds. Vascular tissue isolated from rats was used as a control. We hypothesized that birds lack RAGE as they do not suffer Type II Diabetic pathologies. Our data indicate a band at 55kda suggesting the presence of a RAGE in birds. The density of these receptors in the vasculature is to be determined by immunohistochemistry. E. Cousens was a recipient of an APS UGSRF.