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Diosgenin suppress cardiac apoptosis and enhance survival pathway in ovariectomized rats
Author(s) -
Lee ShinDa,
Tasi ChingYi,
Ou HsiuChung,
Huang ChihYang
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.978.22
Subject(s) - diosgenin , ovariectomized rat , apoptosis , tunel assay , endocrinology , medicine , fadd , fas ligand , caspase 3 , pharmacology , caspase , chemistry , programmed cell death , estrogen , immunohistochemistry , biochemistry , organic chemistry
Objective The study was to evaluate the effects of diosgenin on cardiac apoptotic and survival pathways in bilateral ovariectomied animal model. Methods Sixty female Wistar rats at 6–7 months of age were divided into sham‐operated group (Sham), bilateral ovariectomized group (OVX) for 2 months, and OVX groups with 10 mg/kg and 50 mg/kg diosgenin daily (Ovx 10, Ovx 50) in the 2nd month. The excised hearts were measured by heart weight index, hematoxylin‐eosin staining, positive TUNEL assays, and Western blotting. Results Diosgenin decreased OVX‐induced cardiac TUNEL‐positive apoptotic cells. Diosgenin decreased OVX‐induced Fas ligand, Fas death receptors, Fas‐associated death domain (FADD), activated caspase 8, and activated caspase 3 (Fas pathways). Diosgenin decreased OVX‐induced Bax, Bad, Bax‐to‐Bcl2 ratio, activated caspase 9, and activated caspase 3 (mitochondria pathway). Diosgenin increased ER‐beta, IGF1,IGF1R, PI3K, and Akt(survival pathway). Conclusions Diosgenin suppressed ovariectomy‐induced cardiac Fas‐dependent and mitochondria‐dependent apoptotic pathways and enhance IGF‐1 related survival pathway. The findings may provide one of possible therapeutic approaches of diosgenin for potentially preventing cardiac apoptosis after bilateral ovariectomy or post‐menopause.