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The tempo of cardiovascular and metabolic responses to fasting is different between lean and obese mice
Author(s) -
Tanner Jason,
Kearns Devin,
Kim Bum Jun,
Sloan Crystal,
Yang Guangrui,
Jia Zhanjun,
Yang Tianxin,
Abel E. Dale,
Symons J. David
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.978.15
Subject(s) - medicine , endocrinology , leptin , blood pressure , obesity , heart rate , metabolic syndrome , metabolic rate
Laboratory animals commonly are fasted prior to experimentation. Fasting evokes reductions in heart rate (HR), blood pressure (BP), heat production, and oxygen consumption (VO 2 ) in rodents. We hypothesized that cardiovascular and metabolic responses to a 16‐hour (h) fast (16:00 to 08:00) at 22ºC are blunted in obese vs. lean male C57BL/6J mice. Mice that consumed 45% fat (HF) chow for 98±5 days had elevated (p<0.05) body fat (DEXA), serum leptin (ELISA), HR and BP (72 h biotelemetry), and heat production (72 h metabolic chamber), and lower (p<0.05) VO 2 (72 h metabolic chamber) vs. animals that consumed 10% fat (CON) chow (n=16 per group). As anticipated, each variable decreased (p<0.05) during the fasting segment. However, fasting‐induced reductions in cardiovascular and metabolic variables occurred approximately 4 h and 7 h sooner (p<0.05), respectively, in HF vs. CON mice. Despite differences in the tempo of each response between groups, reductions from the fed condition were identical among animals after 16 h of fasting. Thus, 16 h is a suitable fasting duration for studies of cardiovascular complications in murine models of diet‐induced obesity but shorter periods could be confounded by differential rates of metabolic and vascular adaptations in this species. NIHR15HL091493, ADA7‐08‐RA‐164