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Sex‐dependent influence of insulin (regular and fast) on in vivo albumin and protein clearance
Author(s) -
Bingaman Susan,
Huxley Virginia H
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.975.4
Subject(s) - medicine , endocrinology , insulin , albumin , bovine serum albumin , serum albumin , in vivo , homeostasis , chemistry , biology , biochemistry , microbiology and biotechnology
We have shown previously that the mechanisms whereby males and females achieve volume homeostasis differs and that sex influences vascular leak in response to porcine insulin. Whether regular (Novolin R) and fast (Novolog) insulin induce sex‐dependent changes in albumin (bovine serum albumin, BSA labeled with Alexa 594: C‐BSA) and total protein clearance (C‐TP) was evaluated in anesthetized adult Sprague Dawley rats of both sexes from measures of fluorescence intensity and TP in superfusate collected as it flowed off of the mesentery. Data 80–100 min post insulin (or sham) treatment were compared to that collected 0–30 min after BSA A594 injection. As before, while 0–30 min C‐TP did not differ with sex, C‐BSA was greater in males (N=4) than females (N=4) (20 vs. 30 ng/ml, p<0.05 ). For females by 80–110 min Novalin R reduced C‐TP (−29%) without changing C‐BSA and Novolog had no effect. In contrast, in males by 80–110 min C‐TP and C‐BSA were elevated (>2 and >4‐fold) relative to the shams. The fraction of BSA to TP differed with insulin type (3.6%, 7.7% and 4.7% for sham, Novalin R and Novalog, respectively). These sex‐differences in acute response to insulin infer that insulin resistance may also involve sex‐dependent differences in endothelial barrier function. Supported by NIH HL078816 & NASA NNJ05HF37G.