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EFFECT OF TOP‐LOADING A HEMOGLOBIN‐BASED OXYGEN CARRIER (HBOC) ON MEAN ARTERIAL PRESSURE (MAP), ARTERIOLAR DIAMETER AND TISSUE OXYGENATION
Author(s) -
Song Bjorn Kyungsuck,
Nugent William N,
Golub Alexsander S,
MoonMassat Paula F,
Pittman Roland N
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.973.10
Subject(s) - hemoglobin , oxygenation , mean arterial pressure , vasoconstriction , chemistry , oncotic pressure , intravital microscopy , anesthesia , oxygen , hemodynamics , blood flow , blood pressure , medicine , microcirculation , albumin , heart rate , organic chemistry
This study compared the effects on oxygen (O 2 ) transport of two fluids: a HBOC (HBOC‐201, Biopure Corp., Cambridge, MA) and 5.9% human serum albumin (HSA), a non‐O 2 carrying iso‐oncotic solution (volume control). Intravital microscopic measurements were made on the spinotrapezius muscle of anesthetized, male Sprague‐Dawley rats. Interstitial (ISF) PO 2 was measured using phosphorescence quenching microscopy at baseline and following top‐loading infusions of HBOC‐201 or 5.9% HSA. The purpose of the present study was to identify a dose‐response relationship for HBOC‐201, in which increasing concentrations of HBOC‐201 were infused to reach target plasma concentrations of 1, 10, 100 and 300 μM. Increasing [HBOC] resulted in changes from baseline in MAP (100±1 to 145±5 mmHg), arteriolar diameter (97±2 to 85±5 μm) and ISF PO 2 (72±5 to 63±4 mmHg), while HSA infusion did not elicit significant changes in these variables. Although the total blood hemoglobin concentration increased from 13.3 to 14.3 g/dL due to the addition of HBOC‐201, the decreasing ISF PO 2 is believed to be caused by a reduction in blood flow, and therefore O 2 delivery, subsequent to arteriolar vasoconstriction.