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Long term effects of gamma‐tocopherol nebulization in cutaneous burn and smoke inhalation with early excision and skin autografting in sheep
Author(s) -
Yamamoto Yusuke,
Enkhbaatar Perenlei,
Kraft Edward R,
Rehberg Sebastian,
Sousse Linda E,
Traber Lillian D,
Traber Daniel L
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.970.5
Subject(s) - medicine , anesthesia , smoke inhalation injury , smoke inhalation , inhalation , weaning , airway , oxygenation , lung , surgery
We have previously reported that airway nebulization of gamma tocopherol (g‐T) had a beneficial effect in ovine model of acute respiratory distress syndrome. We hypothesized that nebulization of g‐T into the airway will effectively improve long‐term pulmonary function and wound healing following acute lung injury. Method Ten ewes were subjected to 20%TBSA, third‐degree flame burn and smoke inhalation (36 breaths of cool cotton smoke) under deep anesthesia. One g of g‐T dissolved in 2.2 mL of ethanol was continuously delivered by customized aerosolization device for 48 hrs, starting 3 h post‐injury (Treated group, n=5). Control animals (n=5) were not nebulized. At 24 h post‐injury, escharectomy and skin autografting was performed in both groups. All animals were placed on ventilator and weaning from the ventilator was initiated if PaO2/FiO2 was above 250 at 48 h post‐injury. The sheep were monitored for 3 weeks. Results At 96 h post‐injury, all animals were weaned from ventilator. PaO 2 /FiO 2 (mean±SD) was 505±42 in the treated and 489±47 in the control groups at 3 weeks. Lung bloodless wet‐to‐dry weight ratio (mean±SD) was 2.87±0.16 in the treated and 4.72±1.95 in the control groups (p<0.05). The percentage of the surviving graft area (mean±SD) was 84.2±7.5% in treated and 78.7±5.3% in the control groups. Conclusion The nebulization of g‐T improved long‐term pulmonary oxygenation, reduced lung water content, and increased wound healing following acute lung injury in sheep. Delivery of g‐T into the lungs may be an effective approach in management of burn patients with concomitant lung injury.