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Antihistaminergic effect of a pure compound (GT) isolated from garden slug (Diplosolenodes occidentalis) on tracheal smooth muscle contraction
Author(s) -
Jacob Audrey Shivrani,
Simon Oswald Rudolph,
Wheatle Delano Samuel,
Ruddock Peter Lanton
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.970.1
Subject(s) - histamine , antagonist , chemistry , antagonism , contraction (grammar) , antihistamine , pharmacology , stereochemistry , chromatography , endocrinology , receptor , medicine , biochemistry
Folklore claims of the therapeutic effect of garden slug ( Diplosolenodes occidentalis) extract relieving brochoconstriction in asthmatic individuals were never validated scientifically. Preliminary findings confirmed this effect. The aim of this study was to isolate the compound associated with this effect. Purification of the crude garden slug extract was done by flash column chromatography and Thin Layer Chromatography methods. Structural details were confirmed mainly by Nuclear Magnetic Resonance (NMR) spectroscopic data. The pure compound (GT) was identified as a triglyceride of linolenic acid. In vitro pharmacological studies showed that GT acted in a dose‐dependent manner. Doses of GT, (3.61 x 10 −6 M), (7.20x10 −6 M), (1.4x310 −5 M), (2.9x10 −5 M) and (5.7x10 −5 M) given cumulatively produced statistically significant relaxation ( p<0. 05, n=8) of histamine‐ induced tracheal tension; non‐competitive antagonism was displayed. GT is an antagonist at H 1 receptors since it produced relaxation in tracheal contractions induced by the H 1 agonist histamine trifluoromethyl toluidide dimaleate (200μg).These results classify GT as an antihistamine which acts specifically as an H 1 antagonist. This work was funded by Graduate Studies and Research section of The University of the West Indies