Premium
Phagocyte Immunomodulatory Activity of Oenothein B, A Macrocyclic Elligatannin Isolated from Epilobium angustifolium
Author(s) -
Schepetkin Igor A,
Kirpotina Liliya N,
Jakiw Larissa,
Blaskovich Christie L,
Jutila Mark A,
Quinn Mark T
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.966.2
Subject(s) - phagocyte , proinflammatory cytokine , reactive oxygen species , fucoidan , biology , microbiology and biotechnology , in vivo , cytokine , chemistry , superoxide , biochemistry , immunology , phagocytosis , inflammation , polysaccharide , enzyme
Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. We found that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity‐guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dose‐dependently induced a number of phagocyte functions in vitro , including intracellular Ca 2+ flux, production of reactive oxygen species (ROS), chemotaxis, nuclear factor (NF)‐κB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo , inducing keratinocyte chemoattractant (KC) production and neutrophil recruitment to the peritoneum after intraperitoneal administration. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts. This work was supported in part by NIH grants RR‐020185 and RR‐016455 and NIH contract HHSN266200400009C.