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Radiosensitizing effects of xanthohumol on human cancer cells
Author(s) -
Heo SeWoong,
Kang Youra,
Park MinA,
Kang Keon Wook,
Kim JungAe
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.965.7
Subject(s) - xanthohumol , radiosensitizer , cancer research , radiosensitivity , stat3 , cancer cell , mcf 7 , chemistry , ionizing radiation , cancer , microbiology and biotechnology , pharmacology , apoptosis , medicine , radiation therapy , biology , human breast , biochemistry , irradiation , physics , key (lock) , nuclear physics , ecology
Xanthohumol, the principal flavonoid in hops, was investigated for its radiosensitizing activity on human cancer cells (human breast cancer MCF‐7 and ardiamucin‐resistant MCF‐7 (MCF‐7/ADR) cells, and human colon cancer HT‐29 and HCT116 cells). Cell survival was measured by the MTT assay 2 days after 10 Gy of ionizing radiation. Cells expressing mutant p53 (MCF‐7/ADR and HT29) are more resistant to the ionizing radiation than wild‐type p53 cells (MCF‐7 and HCT116). However, pretreatment of the cells with xanthohumol (10 μM) for 24 h prior to the radiation significantly enhanced radiosensitivity of all the cells, irrespective of p53 status. Some studies showed that there is persistent activation of signal transducer and activator of transcription 3 (STAT3) in a number of human solid tumors including colon cancer. In addition, MCF‐7/ADR showed persistent activation of STAT3. Xanthohumol significantly suppressed the expression of STAT3. In the cells co‐treated with xanthohumol and radiation could have the therapeutic potential as a radiosensitizer in human cancer cells overexpressing STAT3.