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Cytotoxic activity of Taraxacum officinale in OV2008 human ovarian cancer cell line is mediated by caspase 8 activity
Author(s) -
Levy Arkene StacyAnn,
Stupack Dwayne G
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.965.3
Subject(s) - dandelion , cytotoxic t cell , propidium iodide , taraxacum officinale , apoptosis , mtt assay , caspase 7 , biology , caspase , cell culture , cytotoxicity , chemistry , microbiology and biotechnology , traditional medicine , biochemistry , programmed cell death , in vitro , medicine , pathology , genetics , alternative medicine , traditional chinese medicine
The plant Taraxacum officinale, commonly known as dandelion belongs to the family Asteraceae. An aqueous extract of dried Taraxacum officinnale (TO) leaves was evaluated for its cytotoxic activity in OV2008 ovarian cancer cells. Cells were cultured in DMEM media supplemented with 10% FBS. OV2008 cells were seeded in 96 well plates and exposed to TO extract at concentrations ranging from 120–180ug/ml for 72 hours in triplicate. Cytotoxicty was measured using the MTT assay. Significant cytotoxic activity of TO extract was observed in OV2008 cells (IC50 = 140ug/ml). Staining of TO treated cells with propidium iodide revealed the presence of apoptotic bodies. To examine the role of caspases in the apoptotic death of these cells, Caspase 3, 8 and 9 deficient OV2008 cells were prepared via shRNA‐based knockdown of the caspase‐8 transgene. The cytotoxic activity of TO was abolished selectively in caspase‐8 and ‐3 negative OV2008 cells, suggesting that caspase 8 promotes the cytotoxic activity of TO in OV2008 cells. This project was funded by the Fulbright organization and NCI grant CA107263 to DGS.